RNA Details
Disease Name
idiopathic pulmonary fibrosis
Tissue
lung
RNA Symbol
let-7d
RNA ID
RNA Type
miRNA
Alteration Pattern
dysregulation
Species
homo sapiens
Detection Methods
qRT-PCR; Dual-luciferase reporter assay etc.
Target
HMGA2
Pathway
NA
PubMed ID
Title
Let-7d microRNA affects mesenchymal phenotypic properties of lung fibroblasts
Year
2014
Function
"Transfection of primary fibroblasts with let-7d caused a decrease in expression of the mesenchymal markers a-smooth muscle actin, N-cadherin, fibroblast-specific protein-1, and fibronectin, as well as an increase in the epithelial markers tight junction protein-1 and keratin 19. Phenotypic changes were also present, including a delay in wound healing, reduced motility, and proliferation of fibroblasts following transfection. Let-7d transfection significantly attenuated high-mobility group-A2 protein induction by TGF-B. Our results indicate that administration of the epithelial microRNA let-7d can significantly alter the phenotype of primary fibroblasts."